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there is one file with the answers on it and the other one is blank. dont worry about the pictires i just need the answered paraphrased to sounf completely different. it doesnt need to sound smart the more simple the better
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Lab 4 Selective Media & Agar
BIO250L”
Student Name: Click here to enter text.
Access Code (located on the lid of your lab kit): Click here to enter text.
Lab Report Format Expecta ons
U lize college level grammar and professional forma ng when comple ng this worksheet.
Submissions without proper forma ng, all required photos or su cient responses will be rejected.
Pre-lab Ques ons
1. In this lab, you will conduct three di erent experiments that each cover the topic of selec ve
media and agar. How are these experiments di erent from one another? Explain how the
di erent media in each experiment are u lized in the context of selec ve vs di eren al media.
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2. What is the di erence between chemically de ned and chemically complex media? Give an
example (a clinical or environmental research applica on) of when you would use each type of
medium to culture di erent microorganisms.
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3. Why do we grow microorganisms on general growth media rst, before inocula ng di eren al
media?
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4. What changes might you see on a di eren al plate that helps iden fy the bacteria?
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Lab 4 Selective Media & Agar
BIO250L”
EXPERIMENT 1: BIOPROSPECTING FOR STARCH DEGRADING BACTERIA
Introductory Ques ons
1. What are the objec ves of Experiment 1? What media will you use? How does this media
pertain to the topic of selec vity?
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2. What results do you expect to see in Experiment 1 if your selected colony is amylase-producing?
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3. What are some other poten al sources of starch-degrading bacteria?
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4. Why did you perform Step 10, under the Prepare Stock Solu on sec on, in this experiment?
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5. What kind of media did you use ini ally, and why?
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6. What is the purpose of the nal sec on of this experiment?
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Lab 4 Selective Media & Agar
BIO250L”
Data and Observa ons
In the table below, report your observed growth corresponding to each plate. Denote whether there was
growth on the starch plate, whether or not clear areas formed around the colonies that grew.
Table 1: Starch Agar and Gram Iodine Results
Sample
Growth on Starch Agar?
(Yes/N)
Clear area around colonies?
(Y/N)
A
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B
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C
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D
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Provide a clear, high resolu on photo of your plates a er incuba on with the lids removed. For credit on
this lab, your photo must show the growth that is reported in your tabulated data, must depict the
correct media with the iodine added and must also include your handwri en name in the background.
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Plate C
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Plate A
Plate D
Lab 4 Selective Media & Agar
BIO250L”
Lab 4 Selective Media & Agar
BIO250L”
Results and Discussion
1. Describe the results of your starch test.
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2. Do the plates contain starch degrading bacteria? How do you know?
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EXPERIMENT 2: SELECTION AND DIFFERENTIATION OF GRAM POSITIVE BACTERIA
Introductory Ques ons
1. What are the objec ves of Experiment 2? What media will you use? How does this media
pertain to the topic of selec vity?
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2. What results do you expect to see from Experiment 2 if the microbes are non-fermenters? What
if they are fermenters?
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3. What chemical in MSA confers selec vity? How?
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4. What component(s) makes MSA di eren al? How?
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5. Why are the nutrient agar plates used in this experiment?
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Lab 4 Selective Media & Agar
BIO250L”
Data and Observa ons
In the table below, report the growth that was observed on each of the plates and their corresponding
source. Denote whether there was growth on the nutrient plate and MSA plates, and then denote the
color of the MSA around the colonies. Also denote any other per nent observa ons.
Table 2: MSA Growth Observa ons
Surface
Tested
Growth (Y/N) –
Nutrient Agar
Growth (Y/N) –
MSA Agar
MSA color around
colonies (Red/Yellow)
Other Observa ons
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Provide a clear, high resolu on photo of your plates a er incuba on with the lids removed. For credit on
this lab, your photo must show the growth and color that is reported in your tabulated data and must
also include your handwri en name in the background.
Lab 4 Selective Media & Agar
Skin/Nose Nutrient
agar
Surface/Control Nutrient
Agar
Skin/Nose MSA
agar
Surface/Control MSA
Agar
BIO250L”
Lab 4 Selective Media & Agar
BIO250L”
Results and Discussion
1. Review the photo of your agar plates. Describe the color around the colonies. Did any of the
colonies change the color of the MSA?
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2. What does the color of the agar tell you about the bacteria taken from each area of your body?
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EXPERIMENT 3: SELECTION AND DIFFERENTIATION OF GRAM-NEGATIVE BACTERIA
Introductory Ques ons
1. What are the objec ves of Experiment 3? What media will you use? How does this media
pertain to the topic of selec vity?
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2. What results do you expect from Experiment 3 if your growth ferments lactose?
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3. What types of bacteria are inhibited on MacConkey agar? What ingredient(s) in MacConkey agar
selects against those bacteria?
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4. What ingredient(s) makes MacConkey agar di eren al?
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5. Look up the formula for MacConkey agar either in a microbiology textbook or online. Is this a
chemically de ned or a chemically complex media? Why is that important?
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Data and Observa ons
Lab 4 Selective Media & Agar
BIO250L”
In the table below, report the growth that was observed on each of the plates. Also denote
Table 3: MacConkey Agar Results
Sample
Growth? (Yes
or No)
Colony Color
(Clear or Red)
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Provide a clear, high resolu on photo of your plates a er incuba on with the lids removed. For credit on
this lab, your photo must show the growth and color that is reported in your tabulated data and must
also include your handwri en name in the background.
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Plate 2
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Plate 1
Plate 3
Lab 4 Selective Media & Agar
BIO250L”
Results and Discussion
1. Describe the growth you observed on the MacConkey Agar.
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2. For each plate, provide an analysis of the results. What does the color of the colonies for each of
the samples mean regarding the interpreta on of the results?
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