Description
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Thin Layer Chromatography
Student Name
Date
Purpose
Procedure
Experimental Data
Table 1: solvent acetone
If you observe more than one band produced by the same compound, report
them separately on two different rows in the table.
Plant
Extract/Dye
Band Color
Distance
Traveled from
Origin (mm)
Distance
Traveled by
Solvent Front
(mm)
Rf Value of
Band
1
Plant
Extract/Dye
Band Color
Distance
Traveled from
Origin (mm)
Distance
Traveled by
Solvent Front
(mm)
Rf Value of
Band
Photo 1
All photos need to be taken as soon as TLC plates are dry. Exposure to air will
cause some bands to change color.
In all photos the bands should be marked and identified.
Table 2: solvent acetone:octanol
If you observe more than one band produced by the same compound, report
them separately on two different rows in the table.
Plant
Extract/Dye
Band Color
© 2016 Carolina Biological Supply Company
Distance
Traveled from
Origin (mm)
Distance
Traveled by
Solvent Front
(mm)
Rf Value of
Band
2
Photo 2
Lab Questions
1) which mobile phase you used in this experiment is more polar, and why?
2) Do some research on the vegetables or fruit you used, and on the
compounds that should be visible with this technique.
3) Did the Rf of the pigments change with different mobile phases? Is that what
you expected? Explain.
4) List some experimental problems that could have occurred.
Experimental Conclusions
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